The Amazing Spider-Man Vol. 2 (1998-2013) #538

Plot

While Spidey is off participating in the final battle of the Civil War, the Kingpin's sniper is playing a waiting game while keeping Peter's family in his sights; With the Civil War over, Spider-Man returns home to MJ and Aunt May and the sniper takes his shot; Peter pushes Mary Jane out of the way but Aunt May finds herself in the line of fire and is shot.