The Amazing Spider-Man Vol. 2 (1998-2013) #541

Plot

Now that he knows the Kingpin ordered the hit that brought down Aunt May, Spidey takes the goon who shot sniper Jake Martino down to the sewers and mercilessly pumps him for information; Returning to the hospital and finding that Aunt May is not getting any better, with their money running out, Peter decides to take drastic measures; He gives Aunt May a transfusion of his own blood as this has helped her recover in the past; Knowing that Spidey is onto him, Kingpin pays off the prison guards to bring him his clothes and open the doors so he can escape; As he is walking out, Spider-Man arrives for a confrontation.